Feufel,M.A.

During the last 3 years the sequencing market has completely changed. New technologies in the so-called next-generation sequencers can produce gigabases of sequence raw data in only a few days. In the past, data production represented the greatest cost factor in a sequencing project. At present, bioinformatic analysis represents a challenge. The main difference between technologies in influencing bioinformatic analysis is the read length. The classical Sanger system produces a read length of 1100 bases on average, whereas that of next-generation instruments is 250-400 bases (Roche/454 GS FLX sequencer) or 36-100 bases (Illumina/Solexa. genome analyzer; Applied Biosystems SOLiD). To ensure the success of a sequencing project and to maximize the information obtained, it is necessary to choose optimal next-generation technology or a combination of technologies followed by bioinformatic analysis in a sequential approach using state-of-the-art analysis tools.

Feufel, M. A. (2013). Ein kurzer "Knigge" über den Umgang mit Risiken und Unsicherheit [A short manual on how to deal with risks and uncertainty]. Gynäkologische Praxis, 37, 519-529.

Publication year	2013
Document type:	Article
Publication status:	Published
External URL:
Categories:	EducationHealthRisk Communication
Keywords:	bioinformaticsnext-generation sequencer.read length